I. Fluorescent tags: Citrine-YFP and CFP
II. Gene tagging
PCR reaction mixture
PCR cycles
PCR reaction mixture (no primers)
PCR cycle
TT-PCR reaction mixture
TT-PCR cycles
BP reaction mixture
BP reaction conditions
PCR reaction mixture
PCR cycles
b. LR reaction and selection and identification of recombinant clones
LR reaction mixture
LR reaction conditions
III. Production of transgenic Arabidopsis expressing the tagged genes
1. Introduction of binary constructs into Agrobacterium
2. Agrobacterium-mediated transformation of Arabidopsis
Bibliography