A researcher investigates the kinetics of the enzyme catalase, which breaks down hydrogen peroxide ($H_2O_2$) into water and oxygen gas ($O_2$). Five separate trials are set up, each containing the same concentration of catalase and maintained at a constant temperature of 37°C and a pH of 7.0. In each trial, a different initial concentration of $H_2O_2$ substrate (0.4 M, 0.8 M, 1.2 M, 1.6 M, and 2.0 M) is added.
The volume of $O_2$ gas evolved is measured at regular intervals over three minutes.The researcher then uses the graph of oxygen volume against time to calculate the initial reaction rate for each substrate concentration.
What is the main dependent variable calculated in this investigation? What is the dependent variable?