Manual "
expectant
gate" parameters
When applying this
manual
gate to other samples,
switch
to an "
AutoGate
gate"
IF
the clusters within it contain
at least
:
>
% of the plot's cells (
POP
).
>
% of their cells in the manual gate (
PIM
).
>
% of the manual gate's cells (
POM
).
"
Note
:
Expectant gating
" enhances how AutoGate applies a manual gate to other samples.
AutoGate applies manual gates according to their proximity to clusters matched between the applied-from and applied-to samples.
Expectant gating then
removes
the manual drawn boundaries
IF
they contain 1 or more clusters in a significantly proportional manner.
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