Microtome Cutting Thick And Thin at Joel Gsell blog

Microtome Cutting Thick And Thin. Blocks are roughly trimmed to save time. Avoid under‐filling the cassette as this can allow unstable clamping in the microtome and lead to cutting “thick then thin” sections and other. Section shows areas of varying thickness (‘washboard’ or ‘venetian blind’ effect, chatter) If the tissue is soft, the section will be cut, but the block face where the next section will come from will be compressed down and forward, abraded, and damaged. In the sledge microtome, the block is fixed in a static position within a steel carriage. The last few sections are always cut at what will be the final thickness to polish the block face.

Rotary Microtome CUT 4060 microTec GmbH
from www.micro-tec.de

If the tissue is soft, the section will be cut, but the block face where the next section will come from will be compressed down and forward, abraded, and damaged. Blocks are roughly trimmed to save time. The last few sections are always cut at what will be the final thickness to polish the block face. In the sledge microtome, the block is fixed in a static position within a steel carriage. Section shows areas of varying thickness (‘washboard’ or ‘venetian blind’ effect, chatter) Avoid under‐filling the cassette as this can allow unstable clamping in the microtome and lead to cutting “thick then thin” sections and other.

Rotary Microtome CUT 4060 microTec GmbH

Microtome Cutting Thick And Thin Avoid under‐filling the cassette as this can allow unstable clamping in the microtome and lead to cutting “thick then thin” sections and other. Blocks are roughly trimmed to save time. If the tissue is soft, the section will be cut, but the block face where the next section will come from will be compressed down and forward, abraded, and damaged. Section shows areas of varying thickness (‘washboard’ or ‘venetian blind’ effect, chatter) In the sledge microtome, the block is fixed in a static position within a steel carriage. The last few sections are always cut at what will be the final thickness to polish the block face. Avoid under‐filling the cassette as this can allow unstable clamping in the microtome and lead to cutting “thick then thin” sections and other.

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