We demonstrate that monocytes differentiated by macrophage colony-stimulating factor (M-CSF) or granulocyte macrophage colony-stimulating factor (GM-CSF) resembled in vivo inflammatory macrophages, while moDCs resembled in vivo inflammatory DCs.
Recently, transcriptomic, epigenetic, functional, and fate-mapping studies established the identity of three mononuclear cell lineages within the myeloid cell network: macrophages (Macs), dendritic cells (DCs), and monocytes (MOs) (Schlitzer et al., 2015a).
We have examined tissue-factor generation by mononuclear cell subpopulations prepared in a variety of ways in order to determine the cell of origin of mononuclear cell TF and the conditions necessary for maximal in vitro TF generation.
Public scRNA-seq data sets from bronchoalveolar lung fluid and our peripheral blood mononuclear cell scRNA-seq data show CD14 + monocytes/macrophages TF transcript expression levels are elevated in severe but not mild or moderate COVID-19 patients.
When an interstitial macrophage niche is empty, classical monocytes can proliferate locally in a manner that is restricted by the transcription factor MAFB, before undergoing differentiation...
