Recombinant Wrn Protein at Virginia Reynolds blog

Recombinant Wrn Protein. Pathogenic mechanisms of some of the missense variants have been investigated using recombinant mutant wrn proteins. Recombinant human werner's syndrome helicase wrn protein is a human fragment protein, in the 1322 to 1432 aa range, expressed in wheat germ. Subsequently, recombinant wrn protein was shown to have 3′ → 5′ exonuclease activity on dsdna with 3′ recessed termini 11. To investigate whether the duplicated region of human wrn affects premature aging, we first delivered the recombinant proteins into wrn. We also needed a method for production of catalytically active recombinant wrn helicase protein that could be scaled up to make.

WRN is oxidized in vitro by iron but not by copper. A, WRN
from www.researchgate.net

To investigate whether the duplicated region of human wrn affects premature aging, we first delivered the recombinant proteins into wrn. Subsequently, recombinant wrn protein was shown to have 3′ → 5′ exonuclease activity on dsdna with 3′ recessed termini 11. Recombinant human werner's syndrome helicase wrn protein is a human fragment protein, in the 1322 to 1432 aa range, expressed in wheat germ. We also needed a method for production of catalytically active recombinant wrn helicase protein that could be scaled up to make. Pathogenic mechanisms of some of the missense variants have been investigated using recombinant mutant wrn proteins.

WRN is oxidized in vitro by iron but not by copper. A, WRN

Recombinant Wrn Protein Pathogenic mechanisms of some of the missense variants have been investigated using recombinant mutant wrn proteins. To investigate whether the duplicated region of human wrn affects premature aging, we first delivered the recombinant proteins into wrn. Subsequently, recombinant wrn protein was shown to have 3′ → 5′ exonuclease activity on dsdna with 3′ recessed termini 11. Pathogenic mechanisms of some of the missense variants have been investigated using recombinant mutant wrn proteins. We also needed a method for production of catalytically active recombinant wrn helicase protein that could be scaled up to make. Recombinant human werner's syndrome helicase wrn protein is a human fragment protein, in the 1322 to 1432 aa range, expressed in wheat germ.

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