Traffic Light Reporter Assay at Charlie Oliver blog

Traffic Light Reporter Assay. To validate these findings, we used the traffic light reporter (tlr) assay for homologous recombination 45. 8 however, this system requires the generation of reporter cell lines and therefore can not be applied easily in primary cells or animal models. The hdr pathway enables precise sequence modifications if a dna repair template is available. B the ‘traffic light’ reporter (tlr) system indicates the ratio of dsb repair by nhej or hdr. Given our observation that cgamp inhibits g1/s phase cell cycle progression, we examined whether cgamp signaling impairs hdr. The traffic light reporter (tlr) assay protocol is a dual fluorescent readout that has the ability to monitor simultaneous homologous recombination.

The traffic light reporter. (a) Diagram of the TLR. Arrow represents
from www.researchgate.net

The hdr pathway enables precise sequence modifications if a dna repair template is available. 8 however, this system requires the generation of reporter cell lines and therefore can not be applied easily in primary cells or animal models. The traffic light reporter (tlr) assay protocol is a dual fluorescent readout that has the ability to monitor simultaneous homologous recombination. B the ‘traffic light’ reporter (tlr) system indicates the ratio of dsb repair by nhej or hdr. Given our observation that cgamp inhibits g1/s phase cell cycle progression, we examined whether cgamp signaling impairs hdr. To validate these findings, we used the traffic light reporter (tlr) assay for homologous recombination 45.

The traffic light reporter. (a) Diagram of the TLR. Arrow represents

Traffic Light Reporter Assay Given our observation that cgamp inhibits g1/s phase cell cycle progression, we examined whether cgamp signaling impairs hdr. Given our observation that cgamp inhibits g1/s phase cell cycle progression, we examined whether cgamp signaling impairs hdr. To validate these findings, we used the traffic light reporter (tlr) assay for homologous recombination 45. 8 however, this system requires the generation of reporter cell lines and therefore can not be applied easily in primary cells or animal models. B the ‘traffic light’ reporter (tlr) system indicates the ratio of dsb repair by nhej or hdr. The traffic light reporter (tlr) assay protocol is a dual fluorescent readout that has the ability to monitor simultaneous homologous recombination. The hdr pathway enables precise sequence modifications if a dna repair template is available.

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