Ghost Dye™ UV 450 is an amine reactive viability dye that can be used to discriminate viable from non. Ghost Dye UV 450 Fixable Viability Dye is used to discriminate viable from non. Ghost Dye™ UV 450 from Cytek is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining.
This reagent has an excitation peak at 342 nm and an emission peak at 432 nm. Ghost Dye UV 450 Apps: FC Size: 100 tests Catalog No.50-105-2990 $52.80 / Each Qty Add to cart Description SDS Documents Product Certifications Promotions Description Description SDS. PRODUCT INFORMATION Contents: Excitation Laser: Emission (nm): Formulation: Ghost Dye™ UV 450 UV (355 nm) 450 1 uL/test in DMSO DESCRIPTION PREPARATION & STORAGE APPLICATION NOTES Use By: 6 months from date of receipt Mouse splenocytes were stimulated overnight with PMA and ionomycin and stained with Ghost UV 450.
Viable gate is indicated. Ghost DyeTM UV 450 is an amine reactive viability dye that can be used to discriminate viable from non. Ghost Dyes bind irreversibly to amine groups and are resistant to subsequent washing, fixation, and permeabilization, leaving behind a bright and stable fluorescent signal.
On live cells, this class of dye is only able to bind to surface amines. Dead cells with compromised membranes allow the dye to permeate and bind amine groups of intracellular proteins within the cytoplasm. This results in.
Ghost Dye™ Violet 450 is an amine reactive viability dye that can be used to discriminate viable from non. Ghost Dye ™ UV 450 Fixable Viability Dye is used to discriminate viable from non-viable mammalian cells in flow cytometry applications. Ghost Dye ™ UV 450 Fixable Viability Dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes.
Non. Ghost Dye ™ UV 450 Fixable Viability Dye is used to discriminate viable from non-viable mammalian cells in flow cytometry applications. Ghost Dye ™ UV 450 Fixable Viability Dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes.