Sun Dye Qpcr

SUN fluorophore is a molecular equivalent to VIC. SUN dye provides high signal intensity and comparable performance to VIC in qPCR assays. SUN Probes From IDT Demonstrate Superior Results to VIC Probes in qPCR This technical note talks about the role of Fluorophore/Dye in a polymerase chain reaction (PCR).

The fluorophore in absolute PCR quantification acts like a label that helps measure the amount of deoxyribonucleic acid (DNA) being amplified. Objective: The SUN fluorophore is a molecular equivalent to the VIC fluorophore1. The SUN fluorphore is thought to offer signal intensity that is either comparable or superior to dyes with same excitation (538 nm) and emission (554nm) spectra1.

To evaluate how the SUN fluorophore performs in comparison to the VIC fluorophore, we carried out qPCR experiments that targeted the Beta-Actin (ACTB. The experimental design for multiplex qPCR is more complicated than for single reactions. The probes used to detect individual targets must contain unique reporter dyes with distinct spectra.

The settings for excitation and emission lters of real-time detection systems vary from manufacturer to manufacturer; therefore, instruments must be calibrated for each dye as part of the experiment. Dye Substitutes Several qPCR instruments utilize proprietary dyes which are not generally available commercially, such as VIC ® and NED™. When seeking dye alternatives, the following criteria are important: The excitation and detection wavelength are compatible with the instrument light source and detection system.

Search all FAQs: Does IDT offer VIC ® probes for qPCR? IDT offers the SUN™ dye, which is our product offering for the VIC dye (Thermo Fisher Scientific). More information about probes labeled with the SUN fluorophore can be found on the PrimeTime™ qPCR probes page or the SUN fluorophore Decoded™ article. Four dye options optimized for better sensitivity TaqMan QSY probes can be ordered with FAM, VIC, and our proprietary ABY and JUN dyes, allowing amplification of up to four targets in a single reaction.

All four dyes are optimized for the filter sets on Applied BiosystemsTM qPCR instruments (Figure 3) and work together with minimal spectral overlap for optimal performance. Choose the right qPCR dye wavelength and dye combinations for multiplex PCR based on emission spectra, excitation wavelength, and instrument compatibility. Learn what fluorophores are compatible with various real.

5' SUN Return to Fluorophores Modifications A VIC® (ThermoFisher Scientific) equivalent (emission maximum of 554 nm) that is recommended for use in multiplexed assays when added to the 5' end of a dual-labeled 5' nuclease probe for qPCR.