Paternity of hatchlings was confirmed usinggeneticanalyses. This is the first documented case of successful artificial insemination usingfrozen-thawedsemen in a reptile and is a significant step forward in the conservation of reptiles.
Sciencebringing new pinesnakeslifeinto the world The Memphis Zoo collected, froze, andthawedsemen to artificially inseminate a female Louisiana pinesnake.

Here, we used the cornsnake(Pantherophis guttatus) as a reptile model species to demonstrate that the shed skin is a high-quality DNA source for genome sequencing. Skin sheds provide a noninvasive type of sample that can be easily collected without restraining or harming the animal.

Memphis Zoo has successfully produced the first-ever reptile offspring using cryopreserved (frozen) semen and artificial insemination—a major leap forward in reptile conservationscience.

We fed thesnakesonce each week usingfrozen-thawedfish and rodents for N. fasciata, rodents and lizards for C. constrictor, and lizards for T. saurita. We checked terraria daily and collected fresh feces while avoiding urates and portions of feces comprised of mostly fur remnants.
Unlock Your Reptile's Genetics—One Shed at a Time. No popping, no probing—just a safe, lab-verified shed test. Discover your reptile's sex and hiddengeneticswith a simple skin sample.
Here we test whether the DNA in dried shed skin is suitable for reduced representation sequencing approaches, specifically genotyping-by-sequencing (GBS).