Whole transcriptomic profiles and pathway of islets in diet-induced diabetes. Islets obtained from C57BL/6Jmice fed a 60% HFD or a CD for 24 weeks were isolated for whole transcriptome sequencing (Fig 2A).
Subsequent transcriptomic analyses of islets and liver unveiled the chronological order of molecular events during the deterioration of pancreatic islet function.Integrative transcriptomic analysis of pancreatic islets from patients with prediabetes/type 2 diabetes.
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We describe a method for the isolation of endocrine cells from embryonic, neonatal and postnatal pancreases followed by single-cell RNA sequencing. This method allows analyses of pancreatic endocrine lineage development, cell heterogeneity and transcriptomic dynamics.
By Rohan Khanna , Research Fellow , Pine Biotech. Omicslogic Learn Profile - https://learn.omicslogic.com/user/JVjzG8a3PdZhNLONj0EAOu4FQMD3...
We compared the transcriptomic differences between patients with HCM and control populations across three different datasets, and further screened through clustering and enrichment analysis, to identify hub differential expressed ion channels (DEICs) in HCM progression.
Overall, transcriptomics analysis revealed 408 (397 + 11) DE genes that distinguish the islets from KD-fed mice from islets of mice fed either HFD or LFDthat is expression of these 408 genes were significantly different in KD versus both LFD and HFD conditions (Fig. 6A).
Our flow cytometric analyses of dispersed islets from either NCD or HFD mice did not reveal any population of adaptive immune cells (Figure S3A). To further confirm this, we performed immunostaining of frozen pancreatic sections from both NCD and HFD mice.
