Immunofluorescence staining showing GLP
The numbers of GLP-1 + cells and glucagon + cells were manually counted and the ratio was calculated for 25-30 islets in each group.
(A) Immunofluorescence staining showing GLP-1 expression in pancreatic islets. Normal human pancreatic slices were co-stained with anti- glucagon and antiamidated GLP-1 7-36 antibodies.
Expression Profile of the GLP
GLP -1R localization in mouse pancreas. Representative microscopy images of mouse pancreatic islets sections stained with antibodies specific for GLP -1R (A,B,C) (brown DAB stain, n = 5) and insulin (INS, red) (A), glucagon (GCG, green) (B), or somatostatin (SST, red) (C) showing variable degree of overlap. Inserts show cells with overlapping staining at higher magnification. GLP -1R ...
Technical Support Center: Optimizing GLP -1R Immunofluorescence This guide provides troubleshooting advice and answers to frequently asked questions to help researchers and scientists optimize fixation and permeabilization for the immunofluorescent staining of the Glucagon -Like Peptide-1 Receptor ( GLP -1R).
Reassessment of antibody
There is tremendous interest in understanding how and where glucagon -like peptide 1 (GLP-1)-based medicines exert their direct actions, including reduction of inflammation. 1 Localization of many G protein-coupled receptors (GPCRs), including the GLP -1R, at the protein level is challenging due to low-level expression and the scarcity of rigorously validated antibodies. In the intestine, mouse ...
Glucagon -like peptide 1 receptor ( GLP -1R) agonists exhibit anti-inflammatory actions, yet the importance of direct immune cell GLP -1R signaling remains uncertain. Although T cells respond to GLP-1 , low receptor abundance and suboptimal antisera complicate efforts to characterize immune cell GLP -1R signaling. Here, we evaluate three frequently utilized GLP -1R antibodies, revealing that several ...
PDF Application Notes and Protocols for Immunofluorescence Co
These application notes provide a comprehensive guide for the simultaneous detection of insulin and glucagon in pancreatic tissue sections using immunofluorescence . This technique is critical for studying the morphology of islets of Langerhans, the relative distribution of α- and β-cells, and assessing the effects of therapeutic interventions on islet health and function.
The resulting sensor variant showed good membrane expression and a 528% maximal fluorescence response upon GLP-1 binding (Figure 1b-c, Figure 1-figure supplement 1h). This final variant was named GLPLight1 and was chosen for further characterization.
Revealing the tissue
Here the authors generate mice with an enzyme self-label genome-edited into the endogenous glucagon -like peptide-1 receptor locus, design fluorescent dyes for specific labelling in complex tissue ...
Glucagon -like peptide-1 receptor is an important regulator of appetite and glucose homeostasis. Here the authors describe super-resolution microscopy and in vivo imaging compatible fluorescent ...