Effect of Repeated Freezing and Thawing on 18 Clinical Chemistry ...
The organ samples were divided into two separate groups, based on the shipment time. The groups have been referred to as 'thawed' and 'frozen' (n1 = 8 9 5 and n2 = 7 9 5, respectively). All samples were stored at -80 C until metabolic profiling analysis.

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Effect of repeated freezing and thawing on 18 clinical chemistry ...

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Our study demonstrated that a rat frozen shoulder model using immobilization generates the pathophysiologic process of inflammation leading to fibrosis on the glenohumeral joint similar to that seen in patients with frozen shoulder.
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