Therefore, to promote the formation of the correct disulphide bonds in E.coli -expressed hEK L, we employed three strategies: (i) use of a trxB, gor, ahpC*+ mutant expressing cytoplasmic DsbC (SHuffle strain) for oxidative folding, (ii) replacement of the free cysteine with serine (C112S), which bound to heavy chain, to reduce misfolding ...
As we can see from the illustration, Ek Expressed Formations ... has many fascinating aspects to explore.
