What began as a one-off in 2005 as Ten Simple Rules for Getting Published [1] has, in thirteen years, now multiplied a hundredfold to become One Thousand Simple Rules for many aspects of one’s professional development and led to Quick Tips in the journal’s Education section. This milestone of a thousand rules has been reached thanks to the unselfish work of all stakeholders—authors, editors, reviewers, and readers. Let’s face it, writing, editing or reviewing a Ten Simple Rules (TSR) article is not the same as a publication that advances a scientific field. What it is going to get you is the satisfaction of knowing you have passed on a part of your experience in a form that is easily understood and acted upon by those following in your footsteps and hence have a very different kind of positive impact on science. Thank you. On the other hand, as a reader the TSRs might contribute in some small way to you getting tenure, or whatever else you care about in your professional life.

These are our words. In the summer of 2018 PLOS sent out a survey to both the authors and readers of TSRs to solicit your thoughts on the series. A summary of some of your words is presented in Table 1 and Table 2.

The story of how the TSRs started was outlined in that first article [1]. Suffice it to say here that the articles proved then, as now, a succinct and easily digestible piece of advice. Qualitative measures of success of the TSRs appear as framed versions on the walls in labs, or in the case of one of us (PEB) a stand in the corridor outside the lab which contained the collection of TSRs which periodically needed to be replenished. The fact that the stand was located near the bathroom and was eventually stolen will be left unexplored. The definitive qualitative measure of success came with the “Ten Simple Rules for Writing Ten Simple Rules” [2].

A more quantitative measure of success comes from the growth of the series (Fig 1) and the article level metrics (ALMs) that PLOS keeps on each article. As of October 2018, there have been a combined total of 8.3 million views and downloads of the TSRs from the PLOS and PubMedCentral (PMC) Websites. Twelve articles each have over 100,000 views and downloads with “Ten Simple Rules for Writing a Literature Review” [3] topping the list with a staggering 1.1 million combined views and downloads. While not consistently translated, various articles have been made available in at least Spanish, Japanese, Russian, Norwegian, Chinese, and Farsi.

It’s hard to capture the breadth of what has been covered in these one thousand rules (Fig 2). Perhaps the best way is with Ten Rules, well actually ten ways to subclass the one hundred articles. Those divisions are:

With this level of coverage, has the series run its course? Is there nothing new to say? A question we have asked at PLOS a number of times only to have it answered by a new and novel submission. Certainly with one thousand rules novelty becomes an issue. Then again, in just reading responses to the reader survey which asked for novel TSR suggestions new ideas emerged—unique features of working for an NGO such as a non-profit foundation; reducing your carbon footprint while doing research; lab management; ensuring a work-life balance—and so on. Moreover, technologies, protocols, expectations, how we conduct science—think social media—are changing rapidly which calls for topics to be refreshed. In short, there would seem to be no end in sight.

There are also the questions of scope and form of dissemination. Many of the rules are not confined to computational biology and are clearly read by those outside our field. Since 2005 social media has become mainstream and while articles get tweeted are they more appropriate as blog postings? For now, we (PLOS and the TSR Editors), based on comments received through the surveys, feel we should keep the series going as is, but as a community journal your ongoing input is critical. What do you think comes next?