PLoS Genet plos plosgen PLOS Genetics 1553-7390 1553-7404 Public Library of Science San Francisco, CA USA 10.1371/journal.pgen.1010125 PGENETICS-D-22-00266 Correction Correction: PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development in Arabidopsis thaliana Zhang Feng Tao Wenqing Sun Ruiqi Wang Junxia Li Cuiling Kong Xiangpei Tian Huiyu Ding Zhaojun 15 3 2022 3 2022 18 3 e1010125 2022 Zhang et al This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Correction: PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development in Arabidopsis thaliana

There is an error in panel D of Fig 4. The authors have provided a corrected version of Fig 4 here.

10.1371/journal.pgen.1010125.g001 <italic>PRH1</italic> is regulated by <italic>ARF7</italic> at the transcriptional level.

(A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A. thaliana leaf protoplasts. The left hand panel is a schematic of the effector (35S::ARF7) and reporter (PRH1pro:LUC) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly (P<0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S::MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly (P<0.01, P<0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro:AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL aureobasidin A (AbA). The empty vector pGADT7 was used as a negative control.

 Reference Zhang F, Tao W, Sun R, Wang J, Li C, Kong X, et al. (2020) PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development in Arabidopsis thaliana. PLoS Genet 16(2): e1008044. https://doi.org/10.1371/journal.pgen.1008044 32032352