Molecular grade water Forward TTGTTATTCCGCTCTGCGTAACTGCAGTCGACGGGCGTACCATACGTTGTGGCTGCCGGT GAGTACACGTGTGTAACGTACCTCTTGGTTCTGAACTGGCGGGGAAACTTGTGCTTATAA AGGATAAGCCCTTACGGGGAAAGATTTATCGCCAAGAGATCGGCCCGCATCTGATTAGCT GGTTGGGACGGTAATGGCCTACCTAGGCGACGTGGCAAAGCTGGTCTGAGACGATGATCA GACGCATTGGGACTGAGACACCCTGAAACTCCTACGGGAGGCAAGCCACGGAAATATTGG ACATAGGGGCAACCCTAATTCCGACATTCCGCGTGAGTGATGCCCGCCCTAGGGTTGTAA AGCTCTTTTGTGCGGGAAGATAATGACGGTACCGCAAGAATAAACCCCGGCTAAGTTCGT GCCATCAGCCGCGGTAAGCGAAGGGGGCTAGCCTTGCTCTGAATCCCTGGGCGTAAAGGG TGCGAAGGCGGGTCTTTAAGTCAGGGGTGAAATCCTGGAGCTCAACTCCTGAACTGCCTT TGATACTAAAGATCCTGAGCATCCCAGAGGTGAGTGGAACTGCTTCATTTTGAGATGGAA TTCGTAGATATTGACAAGAGCGCGAAGGGCTAGAGCGGCTCACTGGCTCTTCACTGATGA TGAGGCACGAAAGCTTGGGGAGCACACACGATTAGATACCCTGGGTAGGCCTGGGCGCTA AACCAGGTGTGCGAACCCTTGGGGGGCACACTCGCAAGTGGAGCAGCTAACGCTAGACGC CTTCCGCCGGGGGCGTACGGGCATAGGAATAAAATTCACAGGAATCGAGGGGGGCCCGTC AACCGCGGAACCTGAGGATATTATCAAACGCACGACCGGACCCTGCTGGCCCTTGAATGT TCCAGTCCAGTACAGAAACTGAGCTAGTCCTATAACCTTGACCATACATGACTACATGCT CTCTTTCAACTTCTCTCCGAGAGACATTTGGATAAATGCGGAGAACGAGCCCAACACCCG TCTGGGTA Reverse TTGTTGTGGCGCTCGTGTAGACTTCCTCCAGTCACGTGGTCCTACCGTGGACAGCTGCCT CCTTGGGGTAGGCCACTTGCTTCGGGTGGAACCAACTCCCATGGTGTGACGGGCGGTGTG TACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGGTCCACGATTACTAGCGATTCCAA CTTCATGGAGTCGAGTTGCAGAACCCAATCCGAACTGAGAACCACTTTTGAGAATTTCGA TGACCCCCCCCTTTGCATCCCATTGTGACCGACCTTTTAACGCGTGGGTAAGCCAGACCG AAAAGGGCGAGAGGAATTTAACTCATCCCCCCCTTTCTCGCGGCTTATCACCGGCAGGCT CTTTAGAGTGCCCAACTAAATGGTATCAACTAAAGACAGGCGTTGCTCTCGTTGCTGAAC TTAACATACCATCTCACGACAAGAGCTGATGACGGCCATGCAGCACCTGAGCTCCAGGCT CCGAAGATAAGGCTCCCCATGTGGTGAAGGTCCTGGACATGTCAAGGTCTGGTAAGGTTC TGCGCGTTTGCGTTCAAATAAATCACATGCTCCACCGCTTGGGCGGGGGCCCCGTCAATT GCTTTGAGGTTAACCATGCGACCCCTACTCCCCAAGCGGGAATGCTTTAAACGTTTTGCT GCCCCACTTGTGAGTAAACAACGATCGGCTGAGCATTCACCGATTATTGCAGTGCACGAC CTGGGGATCTAATCCTGTTTGCTCGCCCCCAGGCGTGCCACACAGACGTATGAGCGGGTG AGAGCCCTCTCCCCCTGGCTGGCTTGCGAATATCACGGATTCTCCACTACTTACGGGGCC TCCCATCCCCCCTATACTCAGAAGTTACTATTAAAGTAGGTCCAGAGGGAGAGCCTGGAT TATCCCCATGACAAAAAAACAGCCAACTACTCCTCTTTTCCATTGATTTTTAATTATTAT GAGCTCCATTCAATTTCCTCGGGGGTCGGTAGAAAATAGGGGCGGGGTGCATTTCTGCCC CGCTAC Molecular grade water, through Qiagen DNA extraction Forward GTCGGTCGACTACCTACACTGCAGTCGACGGAAGGCCCTGCTTTTGTGGGGTGCTCGGGT GGCGAAGGGGTGAGAAACACGTGAGTAACCTGTACTTGACTTTGGGATAACTCTTCGAAA AAGGGGCTAATACCGGATAGGAGCTCCTGCTGCATGGTGGGGGTTGGAAAGTATCGGCTG TTGGGGATGGATTCGTGGCTCCTCAGCTTGTTGGCTGGGTAGTGGGGTAACGGCGTTTTG ACGGGACGCCGGCCTGAGAAGGTGACCGGGTGATTTGGGACTGAGATACTGACCAAACTC CTACAGGAGGCCGCGAGGGGGAATTTTGCAATATGCCGGGAAGCCCGATGCCCCAACGCC GCGTGCGGGATTAAGGCCTTCGGGTAGTAAAACCCTTTCGGCTGGGACGAAGCGTGAGTG ACCGAAATGGGTACAGAAGCACCGGCTACTACATGCCTCCGAAAGCGGCGATACGTAGGG TGCGAGCGGTGTCCTGATTTCGTGGGCGGCAGCCTCGAAGGTGGTATAGTTGTACGAAGT GCGTCTCGGGGCTTAAAGTCTGACGTGCTTTCGCACCGCCCTACCGTGAAGAAAGTTTGG AAACATGGAAACTCTAGTGCAGAGGAGGAAAGTGTAATTCTTTGTATGGCAGTCAAATGC GCAAAGATATGGCCGAACCCTAATCTGACACTGCGAATTTCAAGGCCTGTAGGTGATATT GAGTTTTTGAACTCGTGGGGGTCCATCCTGTATACGATACGGTAGTAGGTCTCGGAGCCA ATCCATCGGTCCCGGTGTTACGGAGTTTACAGCGCGCTTCCGGCCCGGGGAACAACAATA GCAATTCTGACCCGGCTAGAAGATGCAAGGGCTAAACAAAACCGGCAGAGGTGGTAACCC CCTTCATCTCGTGGCGAGAAGTTTACTCGCAGTTAGCTCGGATCAGCCAAACAAACAGAG CGTGGCTCCACCCCCTGGATCAGGGCGTCGCGGGCTGACTGGGGAAGGAACGAGTGATCT TTGTCAGTTTACGCTCACGTCCGTTCACCAATGAGGAGATTTCTTCCCACTACCCCAGGG ACGGCATCTACCCTACATGGAAGTTTACATGCTCGGGTCTCGCTTCCGGATGAATCCGAT TGGCCAATTCATTGCTTGAAATTTAGCAGGGTGTCCTGC Reverse TTGTACTCTCTGCTCATGTACGACTTAGTCCTAATACCAGTCCCACCTTCGACGGCTCCC CCACAACGGTTAGGCCACCGGCTTCAAGGTGTTACCAACTTTCATGACTTGACGGGCGGT GTGTACAAGACCCGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACT CCAACTTCATGAGGTCGAGTTGCAAACCCCAATCCGAACTGAAACCGGCTTTCCGAGATT CGCTCACCCTCCCAGGCTCGCCACTCTTTGTACCACCCGTTGTAGCGTGGGGGAAGCCCT GGAAAAAAGGGGAATGATGACTTGACGGCATCCCCCCCTTCCTCCGAGTTGACCCCGGCG GTCTCCACTAACTCCCCACCATAACGTGCTGGCAACAGTGAACAATGGTTGCTCTCAGTG CGGGACTTAACCTACATCCTCAACACGAGCTGACAAAACCCATGCTCCACCTGTGAACCG ACCCCAAAAGGGGCGAACCCATCTCTGAACACTGCCGAGCAATGTTAAACCCGGGTAAGG TGCTCCCCGTTGCATCAAATTAATCCGCAAGCTCCGACGCTTGTGCGGGGGGCCGTCAAT TCCTTTGTGTTTTAAAATTGCGGCCCCACTCCCCCGGGGGAGGGATTTATAATTTTATCT CTAGCGCGGAAACCCGGAAATGGATCCCGCACCTCGTTCCCCCCACGGTGAGGGGGGGCC ACCAGGGTATATTTGTTTTTTGATCTCCCCGCACTCTCACACCTTACGGGGTGGGGAAGG CCAGACTTCCGCTATCCACTGTTTTTCTTCCCTCATATAACTCCTCACACCGCTCCCCCA GGAATGATTGCCCTCCCCCCTTGGGTAAGGGTGCCACGGCGGATAGAACGCTCGTGATGG GTTGACCCGATTAATTCGTAACAAAGAAACCACCCCCCACGCTCCCCTTATGCCAAATTC TATTCAAGACTCCCCGCCCACCCATTTAAAAATTCGCGGGGGTGGCGGCGTAGGGCGAGC CTGCGGCACATAGACAGAGATACCACTACTACTGTCTACTCGCAAAGGAATGTGTGATCC AAAACGCAAGCTCCCACTCGACAGTAGTGTGTAGTCGTACTCTTTGACCTCTTGCCGCGT TATTTCTAGTATCCATAGCTACCAGTCAATTGATCGAGAGTCCGTGCGATATAAAAATAT GACGTACAGATTAGTTTTAGA RBC derived MV pellet Forward GTCCAGTCTCTATAGTCCCTGCAGTCGACTAAACACGTTGAGCTGAGCAGTGGCGATGGG GTGAGTCGGACGGGTGACCCTTCCCTCTCCTGCGGGATTGTTGCGGGTAATAGCAGCTGG ACCTCATACTACCTGCGGGTGAAAGCGGGGGATCGCAAGACCTCCCGCAATTGTAGCGGC CGATGTCTGATTAGGTAGTTGGTGAGGTAAAGGCTCCCGGATCCGACGATCTGTGGTTGG TCTGAGAGGACTACCCGCCACGGAGGGACTGAGACACTGGACAGACTCCTACCGGAGGCA GCCGTGGGGAATTTTGGACAATGAGCGCAAGCCTGATCCAGCCATCCCGCGTGCGGGAAG AAGGCCTTCGGGTTGTAAACTGCTTTTGTAAAGGAAGAAAAGGTGCTGGCTAATACCTGG GACTCATGACGGTACCTGGAGAATAAGCACCTACTAACTACCACCGGCTATCTTCGGTAA TACGTAAGGTGCAATCCTTAAATCGGAATTACTAAATCGTAAAATACTGGCTGGCAGTTA TGCAAGACAGAGGTGAAATCTCCCAGGCTCAGCCTGGGAACTGCCCTCTGTGACTTGTGG ATAGATTACGATATAGGGGGATGGAATTCCGCGTGTAGCATTGAAGTGGTTACATATGCA GAGGAACACCTATGGCGAACGCTATCCCCTGGACGTGTACTGACGCTCATGCACTAAAGC TGAGGGAGCAAACCGGACTCCATACCCTGGAAGTCCACCCCCGGAACTATGTCCACTGGA TGCTGCGACCGTTTCGTCTCGCTCACGTAGATAACCCGTGTAGATGATCAACGTGGGGAG TACCGCCCCCGGGGAGAAACTTCGAGTGAACTGAGGCGCACCCGAATTCGCGGGGGATCA TTGAAGTTTTATGCGTCACACTCAAAAACCCCTTCCTTACCCTCTAACTGTCTAGAATTC CGAATGACGTGTAGGTCTCCTCGAATGAATCGGGGACCTGAGGGTGCAGTACCGCAGGTC GTCTACCTCGCCGGCTGAAGATCTTGGGATTAGTACAACACGTATTGTCCCCCCATTCCA GTTGATACTCCTAATGGGCGTGTCTACTCGAACATGCCGCGCACATAGCTCGGAGACAGC CTGGTATGACCCCTAAGTCCAGCATTGCCCTCTACGGCTAGAGACTTAGAGCCCGTTGAT ACATGCCATGTCACGAGGCGTCTCATGTCTGCGACTGGTGGAGACTATACTCAGCAATCG ATCCGAGTAGACGT Reverse AGTGCTCTCGGACGACTGCGACCTATCACGAGCCTGCCGTGGTAAGCGCCCTCCTTGCGG TTAGGCTAACTGCTTCTGGTGTAACCCGCTCCCATGGTGTGACGGGCGGTGTGTATAAAC CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGACTTCATCA GGCGAATTGCAGAATACCATCCGGAATACAAACGGGTTTCTGGAAATTGGTCCACCTCCA GGGGTGGCCGCCCCTCTGTCCGGACCATTGTAGAACGTGTGAAGCCCTGGCCTTAAGGCC CATGAGAACTTGACGTCTTCCCCACTTTCCTCCGGTTGGACCCCGGCGGCTCCCTAGAAT GCCCTTTCGTTGCAAGTAGGGACCAGGGATGCGGGGTTGGCGGGGTTTAGGCCATCATCC CACGACCCGAGCTGACGACCGGAATGCAGCCACTGTGTTCTGGCTCTCTTTTTAGCACTC GCAAATCTCTTCAGGATGAAAGACATCTCAATGGTAGGTAAAGGTTATGGTCTTGCCTTT GATTCAAATTAATCCTCATGCGCCAGCGCGTGGTGCGGGTCCATTTCAATTCGTTTGAGT TTTAGGCTTGCGCTCGTACTGCCGAGGACGTGAACTTAAAGCGATAGCTCTGATAACGAA ACCCTCCAAACCACCAGCTGACCTCTTTTGGGGCGTGGACGATCAGGGTATCTAGTCCTG TTAGCTCTTTTCGCTCCACAGCATGAGCGTCAGCACAGGTACAGGGGCTCGCGAGCCCCC TCGGCCTTCGAGCGCATATCCAGAAATCTCCCTGTTTCACTCCTACTCCGAGGAATTCTC TTCCCACTACAAATATGAGATCATACAGGTTCTTCCGAGAGTCGAGAGGGTGAGGATCCA GCTTTATCTTGTGAACTAAAAAACCCACTCACTACCCTTTTACGTTCCGAATATTCGGAT AACCCTACGACCTACCGAATCGCCCGGCAGGCTGGAGGAAAGTACCCAGGCCTCAGTTAC CTGGTACGTACTAGGAGTCACGAAAAAGGCGACTCCATTTATAACCGCAGTTACGACCTC CTCGGCCCTCCTGCCTTATCCAACCATCTGACACTAGTATGCATCCCTCGACCTAATGTC CAATTGCAGCCTCGCGTCATCTCCTAGGCACATGTGAACACGGCTCCCATGATCAC Streptococcus pneumonia Forward GATTGCTGGCGCGTGCTATACATGCAGTAGAACGCTGAAGGAGGAGCTTGCTTCTCTGGA TGAGTTGCGAACGGGTGAGTAACGCGTAGGTAACCTGCCTGGTAGCGGGGGATAACTATT GGAAACGATAGCTAATACCGCATAAGAGTGGATGTTGCATGACATTTGCTTAAAAGGTGC ACTTGCATCACTACCAGATGGACCTGCGTTGTATTAGCTAGTTGGTGGGGTAAGGGCTCA CCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACAC GGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC CGAGCAACGCCGCGTGAGTGAAGAAGGTTTTCGGATCGTAAAGCTCTGTTGTAAGAGAAG AACGAGTGTGAGAGTGGAAAGTTCACACTGTGACGGTATCTTACCAGAAAGGGACGGCTA ACTACGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGC GTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGT AGGCTTTGGAAACTGTTTAACTTGAGTGCAAGAGGGGAGAGTGGAATTCCATGTGTAGCG GTGAAATGCCTAGATATATGGAGGAACACCGGTGGCGAAAGCTGCTCTCTGGCTTGTCAC TGACGCTGAGGCTCGAAAGCGCGCGGAGCATACAGGATTAGATACCCTGGTAGTCCAAGC TGTACACTATGAGTGCTAGGTGTTAGACCCTTTTCCGGCGTTTAGTGCCGTAGCTAACGC ATTAAGCACTCCGCCTGGGAGTACGACTTAAAAGGTTGAAACACAGCATGAAGTGACTGG AGCCCGCCCAATCAGTTGAGCATGTAGTTAATCCGAAGCACTGTTAAGAACATAACAAGG TCTGAACATCCTCGGACTACTATTAGCACGGAGTTGTCGTTGCAGAAAGAGTGATCCTTG TGATGAATGACATCACACAGGAAGTACAGCTTGCGCTACGTTCCTATGATGCACGTATAT CATACAGTCTCTACTAGGACAGCGAGTGAGTACGTGTATTAACTCGATAGAGTCTCGTCA ATCCGTGCGTCGCACGCTGCTCACCACGACTGATAGACAGACCTGGGTATGATGCCACGA GTATGCACGATCGA Reverse GTTGGTACGAGCTCTTGTTCGACTTCCCCCATCATCTATCCCACCTTAGGCGGCTGGCTC CTTACGGTAACCTCACCGACTTCGGGTGTTACAAACTCTCGGGGTGTGACGGGCGGTGTG TACAAAGCCCGGGAACGTATTCACCGCGGCGATGTGATCCGACATTACTAGCGATTCCGA CTTCGTGTCAGCGAGTTGCGGCCTACAATCCGAACTGAGACTGGCTGGGGAGATTAGCTT GTCGGCGACGCTTGTGACTCTTTGTACCAGGGTGAGTGTACCGTGTGTGTCCCAGGTGAT AAAGGGCGTGAGGATTTTACGCGTCCCCCCCACCCCTCCGCTGTATTACGGCGTCTGACC GAGCTAGTGTCCAAAAAATGATAATGGTATCTGATCTTAGGGGTTGCTCGCGTTGGGGGA CTTAACCCAACATCTCACGACACGCGCTGACGACAACCATGCCCCACCTGGTCACCTCTG TCCCGAAGGAAAACTCTATCTCTAGAGCGGTCAGAGGGATGTCAAGACCTGGTAAGGTTC TTCGCGTTGCTTCGAATTAAACACATGCTCCACCGCTTGTGCGGGCCCCCCGTCAATTCC TTTGAGTTTCAACCTTGCGGTCGTACTCCCGAGGCGGAGTGCTTAATGCGTGAGCTACGG CACTAAACCCCGGAAAGGGTCTAACACCTAGCACTCATCGTTTACAGCGTGCACTACCAG GATATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG AGCCGCTTTCGCCACCGGTGTCCTCCATATATCTACGCATTTCACCGCTACACATGGAAT TCCACTCTCCCCTCATGCACTCAGTTAGACAGTTTCCAAAGCATACTATGGTAGGCACAG CCTTTAACTCAGAACTTATCTAACCTCCTGCGCTCCCCTTACAGCCCAATAAATCCGACA CCTCGGAACTAACATTAACCGCGGTGCTGACATGACTAGCTTCGTTTCAGAAAGATACGT CAGTAACTACATTCAAACCTTCTATCCTAACAAGACTTCATCAGAACCTTACATAGCGTT ACTCTGAGGATCGCACTCGGAGATCCATGCTCCGATCGACTTACACCCGATTCGCAGATC AG