PLoS Pathog plos plospath PLOS Pathogens 1553-7366 1553-7374 Public Library of Science San Francisco, CA USA 10.1371/journal.ppat.1006128 PPATHOGENS-D-16-02789 Correction Correction: Variant Exported Blood-Stage Proteins Encoded by Plasmodium Multigene Families Are Expressed in Liver Stages Where They Are Exported into the Parasitophorous Vacuole Fougère Aurélie Jackson Andrew P. Bechtsi Dafni Paraskevi Braks Joanna A. M. Annoura Takeshi Fonager Jannik Spaccapelo Roberta Ramesar Jai Chevalley-Maurel Séverine Klop Onny van der Laan Annelies M. A. Tanke Hans J. Kocken Clemens H. M. Pasini Erica M. Khan Shahid M. Böhme Ulrike van Ooij Christiaan Otto Thomas D. Janse Chris J. Franke-Fayard Blandine 17 1 2017 1 2017 13 1 e1006128 2017 Fougère et al This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Variant Exported Blood-Stage Proteins Encoded by Plasmodium Multigene Families Are Expressed in Liver Stages Where They Are Exported into the Parasitophorous Vacuole

There are errors in this article that the authors and publisher wish to correct. In Table 2, there are formatting errors in the table headings and in the “Double gene-tagging mutants (multigene families)” section. Please see the corrected Table 2 here. The publisher apologizes for the errors. In addition, in preparation of the figures for publication the authors inadvertently inserted Figure 1 for Fig 3. Please see the correct Fig 3 here.

10.1371/journal.ppat.1006128.t001 Features of tagged members of the <italic>pir</italic>, <italic>fam-a</italic> and <italic>fam-b</italic> multigene families.
Name tagged protein Fluorescent tag Mutant name RMgmDB ID1 BLOOD LIVER
expression and localisation % of clones fluorescent fluorescent before passage (%) fluorescent after passage (%) protein localisation
Single gene-tagging mutants (multigene families)
Fam-a1 mCherry 1477cl3 690 Yes; RBC s 33% (n = 3) 80–90% 50–70% Yes; PV
Fam-a1 GFP 1941 1283 Yes; RBC s N.A. 25–30% 30% No
Fam-a2 mCherry 1448cl2 693 Yes; RBC c pa 60% (n = 5) 99% 85–100% Yes; PV
Fam-b1 mCherry 1599cl4 699 Yes; RBC c pa 66% (n = 3) 5–15% 5% Yes; PV
Fam-b2 mCherry 1731cl4 700 Yes; RBC c pa 100% (n = 5) 65% 50–70% Yes; PV
Fam-b2 GFP 1942 1282 Yes; RBC c pa N.A. 50–75% N.D. N.D
PIR1 mCherry 1531cl3 695 Yes; RBC c pa 100% (n = 5) 15% 60–70% Yes; PV
PIR1 mCherry 1944cl1 1281 Yes; RBC c pa 66% (n = 3) 10–20% N.D. N.D.
PIR2 GFP 603cl3 696 Yes; RBC c pa 75% (n = 4) 5–10% 5% No
PIR3 mCherry 1918cl4 697 Yes; RBC c pa 25% (n = 4) 1%-5% N.D. No
PIR4 mCherry 2450 1233 Yes; RBC c pa N.A. 0.1–2% N.D. No
PIR5 mCherry 2448cl1 1234 Yes; RBC c pa 100% (n = 3) 25–50% N.D. N.D.
PIR6 mCherry 1892 698 Yes; RBC c pa N.A. <0.1% N.D. N.D.
PIR7 mCherry 2211 1235 Yes; RBC c pa N.A. <0.1% N.D. N.D.
PIR8 mCherry 2312, 2313 1236 Yes; RBC c pa N.A. 50–60% 30–60% yes, parasite cyt
Double gene-tagging mutants (multigene families)
Fam-a2Fam-a1Fam-a2/a1 mCherryGFPmCherry&GFP 2010 (2011) 1244 Yes; RBC c paYes; RBC s N.A. 70–80%40–65%40–55% 70–80%45–50%30–40% Yes; PV (>90%)No
Fam-a2Fam-a1Fam-a2/a1 GFPmCherryGFP&mCherry 2504cl3 1245 Yes; RBC c paYes; RBC s 100% (n = 3) 80–90%80–90%80–85% 75–80%60–65%70–80% Yes; PV (70–75%)Yes, PV (30–40%)30–40%
Fam-b1Fam-b2Fam-b1/b2 mCherryGFPmCherry&GFP 2421(-2424) 1246 Yes; RBC c paYes; RBC c pa N.A. 40–50%40–45%35–45% 30–50%50–80%20–40% Yes; PVNo
PIR1PIR3PIR1/PIR3 mCherryGFPmCherry&GFP 2020 (2021) 1247 Yes; RBC c paYes; RBC c pa N.A. 35–45%25–35%20–30% 40–60%1–20%1–5% Yes; PVNo
Single gene-tagging mutants (single copy genes)
IBIS1 GFPmcherry 20091940cl1 1237 Yes; RBC c pu N.A.100% (n = 1) N.D>90% N.D.N.D. Yes; PV (>90%)
SMAC mCherry 1565cl1 1238 Yes; RBC c pa 100% (n = 4) >90% N.D. Yes; PV (>90%)

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 10.1371/journal.ppat.1006128.g001 Maximum likelihood phylogeny of <italic>fam-b</italic> gene sequences from <italic>Plasmodium</italic> spp.

The tree was estimated using RAxML and a GTR+Γ model. Branches subtended by nodes with >75 bootstrap support are shown in bold. Robust basal nodes are indicated by black squares with bootstrap proportions (above node) and Bayesian posterior probabilities (beneath node). At right, coloured blocks indicate the species to which a terminal node belongs. Clades of orthologs that display positional conservation are indicated with green blocks; where a sequence has been lost secondarily in one species, this is shown by an ‘X’. The phylogeny is subdivided into four sections: divergent genes included conserved loci, placed at the root of the tree (below line i); predominantly P. yoelli species-specific genes P. berghei- and P. yoelli-specific paralogs (between lines i, ii and iii); and predominantly P. chabaudi species-specific genes (above line iii). Transcription levels (shown as different coloured and sized circles) in blood stages are shown for individual genes based on RNAseq data (FPKM values) (from [33] and S1 Table). Expression levels as shown by four different sized circles: Class 1 (smallest circle): 2-8x the threshold level; class 2: 8-16x the threshold; class 3 (largest circle): >16x the threshold.

 Reference Fougère A, Jackson AP, Paraskevi Bechtsi D, Braks JAM, Annoura T, Fonager J, et al. (2016) Variant Exported Blood-Stage Proteins Encoded by Plasmodium Multigene Families Are Expressed in Liver Stages Where They Are Exported into the Parasitophorous Vacuole. PLoS Pathog 12(11): e1005917. doi: 10.1371/journal.ppat.1005917 27851824