Look up 3D via sigma difference
This output sets the Z position on its input localizations by computing the difference between the X and Y PSF widths and looking up the difference in the provided the section called “Z calibration file”. Thereby, a Z coordinate can be estimated on data that have been fitted with free PSF widths. For more information, see [Henriques2010].
It is preferable to provide the the section called “Z calibration file” directly to the fitting module by choosing the Interpolated 3D as 3D PSF model. Thereby, a degree of fitting freedom is avoided and precision is enhanced.
Input file name for the calibration table in which the difference is looked up. The format is described in the section called “Z calibration file”.