Bacterial Transformation

graph TD A[Plasmid DNA] --> AND1{Plasmid AND
Cells?} B[Competent cells] --> AND1 AND1 --> Mix[Mix plasmid with cells] Mix --> Chill[Chill on ice] Chill --> Shock[Heat shock 42°C 30-60 s] Shock --> Chill2[Chill on ice 2 min] Chill2 --> SOC[Add SOC recovery medium] SOC --> Recover[Recover 37°C 1 h] Recover --> Plate[Plate on selective agar] Plate --> Incubate[Incubate 37°C overnight] Incubate --> Pick[Pick transformant colonies] style A fill:#ff6b6b,stroke:#c92a2a,color:#fff style B fill:#ff6b6b,stroke:#c92a2a,color:#fff style Mix fill:#51cf66,stroke:#2f9e44,color:#fff style Chill fill:#74c0fc,stroke:#1971c2,color:#fff style Shock fill:#51cf66,stroke:#2f9e44,color:#fff style Chill2 fill:#74c0fc,stroke:#1971c2,color:#fff style SOC fill:#51cf66,stroke:#2f9e44,color:#fff style Recover fill:#74c0fc,stroke:#1971c2,color:#fff style Plate fill:#51cf66,stroke:#2f9e44,color:#fff style Incubate fill:#74c0fc,stroke:#1971c2,color:#fff style Pick fill:#b197fc,stroke:#7950f2,color:#fff style AND1 fill:#b4b4dc,stroke:#8b8bc4,color:#000