Bacterial Transformation

graph TD N1["Competent E. coli"] N2["Plasmid DNA (marker)"] N3["Selective agar + antibiotic"] N4["Mix DNA + cells on ice"] N5["Heat shock 42C, 45s"] N6["Return to ice"] N7["Recover in SOC, 37C 1h"] N8["Plate on selective agar"] N9["Incubate 37C overnight"] N10{"Colonies on plate?"} N11["Transformed colonies"] N12["Check competency/DNA"] N1 --> N4 N2 --> N4 N4 --> N5 N5 --> N6 N6 --> N7 N7 --> N8 N3 --> N8 N8 --> N9 N9 --> N10 N10 -->|yes| N11 N10 -->|no| N12 N12 -->|retry| N4 style N1 fill:#ff6b6b,color:#fff style N2 fill:#ff6b6b,color:#fff style N3 fill:#ffd43b,color:#000 style N4 fill:#51cf66,color:#fff style N5 fill:#51cf66,color:#fff style N6 fill:#51cf66,color:#fff style N7 fill:#51cf66,color:#fff style N8 fill:#51cf66,color:#fff style N9 fill:#51cf66,color:#fff style N10 fill:#74c0fc,color:#fff style N11 fill:#b197fc,color:#fff style N12 fill:#ffd43b,color:#000