DNA Extraction (Phenol–Chloroform)

graph TD N1["Cell/tissue sample"] N2["Lysis buffer + proteinase K"] N3["Lyse cells; digest protein"] N4{"Purification method?"} N5["Phenol:chloroform extract"] N6["Bind DNA to silica column"] N7["Wash column"] N8["Ethanol precipitate"] N9["Elute / resuspend DNA"] N10{"A260/A280 ~ 1.8?"} N11["Purified genomic DNA"] N1 --> N3 N2 --> N3 N3 --> N4 N4 -->|organic| N5 N4 -->|column| N6 N5 --> N8 N6 --> N7 N7 --> N9 N8 --> N9 N9 --> N10 N10 -->|re-extract| N3 N10 -->|yes| N11 style N1 fill:#ff6b6b,color:#fff style N2 fill:#ffd43b,color:#000 style N3 fill:#51cf66,color:#fff style N4 fill:#74c0fc,color:#fff style N5 fill:#51cf66,color:#fff style N6 fill:#51cf66,color:#fff style N7 fill:#51cf66,color:#fff style N8 fill:#51cf66,color:#fff style N9 fill:#51cf66,color:#fff style N10 fill:#74c0fc,color:#fff style N11 fill:#b197fc,color:#fff