ELISA

graph TD A[Antigen or capture Ab] --> Coat[Coat plate 4°C overnight] Coat --> Block[Block with BSA or casein] Block --> Sample[Add sample] Sample --> Inc1[Incubate] Inc1 --> Wash1[Wash unbound] Wash1 --> DetAb[Add enzyme-conjugated detection Ab] DetAb --> Inc2[Incubate] Inc2 --> Wash2[Wash unbound] Wash2 --> Sub[Add chromogenic substrate] Sub --> Stop[Stop reaction] Stop --> Read[Read absorbance in plate reader] style A fill:#ff6b6b,stroke:#c92a2a,color:#fff style Coat fill:#51cf66,stroke:#2f9e44,color:#fff style Block fill:#51cf66,stroke:#2f9e44,color:#fff style Sample fill:#51cf66,stroke:#2f9e44,color:#fff style Inc1 fill:#74c0fc,stroke:#1971c2,color:#fff style Wash1 fill:#51cf66,stroke:#2f9e44,color:#fff style DetAb fill:#51cf66,stroke:#2f9e44,color:#fff style Inc2 fill:#74c0fc,stroke:#1971c2,color:#fff style Wash2 fill:#51cf66,stroke:#2f9e44,color:#fff style Sub fill:#51cf66,stroke:#2f9e44,color:#fff style Stop fill:#51cf66,stroke:#2f9e44,color:#fff style Read fill:#b197fc,stroke:#7950f2,color:#fff