ELISA

graph TD N1["Capture antibody"] N2["Sample antigen"] N3["Microtiter plate"] N4["Coat plate, 4C overnight"] N5["Block with BSA"] N6["Add sample; incubate"] N7["Wash unbound"] N8["Enzyme-conj. detection Ab"] N9["Wash; add TMB substrate"] N10["Stop; read OD450"] N11{"OD above cutoff?"} N12["Analyte quantified"] N13["Reported negative"] N1 --> N4 N3 --> N4 N4 --> N5 N5 --> N6 N2 --> N6 N6 --> N7 N7 --> N8 N8 --> N9 N9 --> N10 N10 --> N11 N11 -->|yes| N12 N11 -->|no| N13 style N1 fill:#ff6b6b,color:#fff style N2 fill:#ff6b6b,color:#fff style N3 fill:#ffd43b,color:#000 style N4 fill:#51cf66,color:#fff style N5 fill:#51cf66,color:#fff style N6 fill:#51cf66,color:#fff style N7 fill:#51cf66,color:#fff style N8 fill:#51cf66,color:#fff style N9 fill:#51cf66,color:#fff style N10 fill:#74c0fc,color:#fff style N11 fill:#74c0fc,color:#fff style N12 fill:#b197fc,color:#fff style N13 fill:#b197fc,color:#fff