Western Blot

graph TD N1["Protein lysate"] N2["Primary antibody"] N3["SDS-PAGE gel"] N4["Denature & load samples"] N5["SDS-PAGE: separate by MW"] N6["Electroblot to membrane"] N7["Block membrane"] N8["Probe primary Ab; wash"] N9["Secondary Ab-HRP; wash"] N10["Add ECL; image"] N11{"Band at expected MW?"} N12["Detected protein band"] N1 --> N4 N3 --> N4 N4 --> N5 N5 --> N6 N6 --> N7 N7 --> N8 N2 --> N8 N8 --> N9 N9 --> N10 N10 --> N11 N11 -->|troubleshoot| N6 N11 -->|yes| N12 style N1 fill:#ff6b6b,color:#fff style N2 fill:#ff6b6b,color:#fff style N3 fill:#ffd43b,color:#000 style N4 fill:#51cf66,color:#fff style N5 fill:#51cf66,color:#fff style N6 fill:#51cf66,color:#fff style N7 fill:#51cf66,color:#fff style N8 fill:#51cf66,color:#fff style N9 fill:#51cf66,color:#fff style N10 fill:#74c0fc,color:#fff style N11 fill:#74c0fc,color:#fff style N12 fill:#b197fc,color:#fff