Description
DNA replication: helicase unwinds the origin, primase/polymerase synthesize the continuous leading strand and discontinuous Okazaki fragments on the lagging strand, which are matured and ligated as the fork advances.
Process Flowchart
graph TD
N1["Origin of replication"]
N2["Helicase unwinds duplex"]
N3["Primase makes RNA primer"]
N4["Pol extends leading strand"]
N5["Lagging: Okazaki fragments"]
N6["Primers removed; gaps filled"]
N7["Ligase seals nicks"]
N8{"Fork continues?"}
N9["Two daughter duplexes"]
N1 --> N2
N2 --> N3
N3 --> N4
N3 --> N5
N5 --> N6
N6 --> N7
N4 --> N8
N7 --> N8
N8 -->|continue| N2
N8 -->|done| N9
style N1 fill:#ff6b6b,color:#fff
style N2 fill:#51cf66,color:#fff
style N3 fill:#51cf66,color:#fff
style N4 fill:#51cf66,color:#fff
style N5 fill:#51cf66,color:#fff
style N6 fill:#51cf66,color:#fff
style N7 fill:#51cf66,color:#fff
style N8 fill:#74c0fc,color:#fff
style N9 fill:#b197fc,color:#fff
Node provenance
Each step below is grounded in a cited source (hover a citation for the full title).
| Step | Role | Source |
|---|---|---|
| Origin of replication | Triggers & Inputs | Alberts, B. et al. 2002 |
| Helicase unwinds duplex | Processing & Operations | O'Donnell, M. et al. 2013 |
| Primase makes RNA primer | Processing & Operations | O'Donnell, M. et al. 2013 |
| Pol extends leading strand | Processing & Operations | Alberts, B. et al. 2002 |
| Lagging: Okazaki fragments | Processing & Operations | Alberts, B. et al. 2002 |
| Primers removed; gaps filled | Processing & Operations | O'Donnell, M. et al. 2013 |
| Ligase seals nicks | Processing & Operations | O'Donnell, M. et al. 2013 |
| Fork continues? | Intermediates & States | Bell, S. P. et al. 2016 |
| Two daughter duplexes | Products & Outputs | Alberts, B. et al. 2002 |
🎨 Color Scheme (5-Color System)
Red
Triggers & Inputs
Triggers & Inputs
Yellow
Structures & Objects
Structures & Objects
Green
Processing & Operations
Processing & Operations
Blue
Intermediates & States
Intermediates & States
Violet
Products & Outputs
Products & Outputs
Process Statistics
- Nodes: 9
- Edges: 10
- Conditionals: 1
- AND Gates: 2
- OR Gates: 2
- Total Gates: 4
Keywords
- dna replication
- helicase
- okazaki
- leading strand
- lagging strand
- ligase
Sources & References
-
Principles and concepts of DNA replication in bacteria, archaea, and eukarya
O'Donnell, M.; Langston, L.; Stillman, B. , Cold Spring Harbor Perspectives in Biology (2013)
DOI: 10.1101/cshperspect.a010108 -
Molecular Biology of the Cell (DNA Replication Mechanisms)
Alberts, B.; Johnson, A.; Lewis, J.; Raff, M.; Roberts, K.; Walter, P. , Garland Science (NCBI Bookshelf) (2002)
View Source -
Chromosome Duplication in Saccharomyces cerevisiae
Bell, S. P.; Labib, K. , Genetics (2016)
DOI: 10.1534/genetics.115.186452