Protein depletion is defined as a strategy aimed at extracting abundant and potentially interfering proteins from a sample matrix to enhance the detection and quantification of low-abundant target proteins.
To obtain an accurate and efficient protein silencing, several strategies have been developed, such as protein displacement systems and targeted protein degradation (TPD) are emerging strategies which aim to achieve loss of function and proteolysis of POI, respectively.
We introduce a cost-effective, robust high-throughputcompatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day.
This particular example perfectly highlights why Protein Depletion Experiments is so captivating.
Summary: We have optimized the conjugation chemistries and blending protocols to achieve >95% depletion efficiency of target high abundance proteins in human plasma samples.
We sought to develop a truly posttranslational protein depletion method based on protein targeting by antibodies. Antibodies bind to proteins with high affinity and specificity; they are widely available commercially and can be produced for almost any protein with relative ease.
