Anitratetestis a chemicaltestused to determine the presence ofnitrateion in a solution. Testing for the presence ofnitratevia wet chemistry is generally difficult compared with testing for other anions because mostnitratesare soluble in water.
Thenitrite(that was originally present plus reducednitrate) is determined by diazotizing with sulfanilamide and coupling with N-(1-naphthyl)-ethylenediamine dihydrochloride to form a highly colored azo dye which is measured colorimetrically.
UseNitrateReductionTestto determine the ability of an organism to reducenitratetonitriteandto identify the different ways thatnitratecan be reduced by bacteria.
NitrateandNitriteView/Download PDF Version What areNitrateandNitrite?Nitrateandnitriteare soluble compounds containing nitrogen and oxygen. In the environment,nitrite(NO2 - ) generally converts tonitrate(NO3 - ), which meansnitriteoccurs very rarely in groundwater.
To determine the levels ofnitrateandnitriteseparately, the procedure is first carried out without the preliminary reduction step in order to quantify the level ofnitritesolely.
Given thatnitriteandnitratehave low concentrations and high water solubility, selecting suitable extraction and clean-up procedures to examine these analytes in biological samples is important. Most reviews have focused on measurement methods used primarily for non-biological samples.
4.1.TestPrinciple Assays ofnitriteandnitratehave become increasingly important in recent years for health care and economic reasons. Whilenitrateis an essential plant nutrient, it constitutes a risk to human health because it plays a part in the formation of methemoglobin and nitrosamines. Negligible amounts ofnitriteoccur in plants and drinking water. However, under unfavorable ...
