Ghost Dye® Violet 510 is an amine reactive viability dye that can be used to discriminate viable from non. Explore fixable viability dyes for flow cytometry with 14 LIVE/DEAD dye options which allow for cleaner separation and identification of cell populations. Flow cytometric analysis of live and heat killed mouse bone marrow cells, combined and stained with Ghost Dye UV 450 Viability Dye.
Viable gate is indicated. Ghost Dyes bind irreversibly to amine groups and are resistant to subsequent washing, fixation, and permeabilization, leaving behind a bright and stable fluorescent signal. On live cells, this class of dye is only able to bind to surface amines.
Dead cells with compromised membranes allow the dye to permeate and bind amine groups of intracellular proteins within the cytoplasm. This results in. Ghost Dye™ Violet 510 from Cytek is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining.
This reagent has an excitation peak at 413 nm and an emission peak at 508 nm. The Live/Dead Ghost dye 780 is a fluorescent dye used to distinguish live and dead cells in flow cytometry. It binds to nucleic acids and emits a far-red fluorescent signal, allowing for the identification and separation of viable and non.
The LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer. Viability dyes help distinguish live cells from dead or dying cells in flow cytometry experiments, and are available in fixable and non.
Overview GloCell™ Fixable Viability Dye Violet 510 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation.