Product Details
IW20001-1
Each lot of this product contains 100 ml PBMC thawing buffer for 11 vials of cryopreserved PBMC.
IW20001-2
Each lot of this product contains 250 ml PBMC thawing buffer for 27 vials of cryopreserved PBMC.
IW20001-3
Each lot of this product contains 500 ml PBMC thawing buffer for 55 vials of cryopreserved PBMC.
Manufactured and Distributed by:
ImmunoWork LLC
605E Huntington Drive #103
Monrovia, CA 91016
TEL: (818) 856-3788
E-MAIL: info@immunowork.com
Store the reagent at 2-8oC. Do not use after expiration.
Recommended Experimental Procedures
1. Aliquot 9 ml PBMC thawing buffer to a 15 ml Falcon tube and warm it up to 37oC in a heat bath.
2. Thaw one vial PBMC (1 ml) in the 37oC heat bath.
3. Gently transfer the thawed PBMC to the warm thawing buffer using a Pasteur transfer pipette.
4. Gently invert the tube twice and then incubate for 10 minutes at room temperature.
5. Centrifuge at 300 g for 5 minutes at room temperature using a swing-out rotor to collect cells.
6. Pour out supernatant.
7. Depending on the downstream application, resuspend cells either in 2 ml of full cell culture media (pre-warmed to 37oC) or flow cytometry buffer (room temperature) using a Pasteur transfer pipette.
8. Resuspend cells gently, and take 100 µl suspension for cell counting.
9. For cell culture: transfer PBMCs to a cell culture flask and supplement with full cell culture media.
10. For flow cytometry assay: place 1 - 2 x 106 PBMCs in a flow cytometry tube containing 1 ml flow cytometry buffer (room temperature).
11. Centrifuge at 300 g for 5 minutes at room temperature.
12. Pour out the supernatant and touch the tube against a Kimwipe to drain the residual buffer. Resuspend cells in the flow cytometry buffer and proceed to the downstream procedures.