Calcium Pantothenate Assay Methods
This document describes two procedures for determining the amount of calcium pantothenate in various pharmaceutical dosage forms using liquid chromatography . The first procedure uses an internal standard and the second does not. Both involve extracting or dissolving the calcium pantothenate and preparing standard and sample solutions for analysis by LC using UV detection. The amount of calcium ...
Abstract and Figures This report describes a fast, simple and reliable HPLC method for the assay and quantitative determination of calcium pantothenate in commercial products.
PDF Calcium Pantothenate Assay USP 2025
The following liquid chromatographic procedures are provided for the pharmaceutical ingredient, a dietary supplement ingredient, or a component While conducting these procedures, protect solutions containing and from atmosphere and light, preferably with the use of low-actinic glassware.
Many dietary supplements contain pantothenate in the form of its calcium salt. "Oil and Water Soluble Vitamins with Mineral Tablets - Calcium Pantothenate " of USP40-NF35 describes two analysis methods using HPLC and one microorganism quantitation method .
PDF Calcium Pantothenate
Calcium phosphate 3-Aminopropionic acid. Examine by thin-layer chromatography (2.2.27), using silica gel G R as the coating substance. Test solution (a). Dissolve 0.2 g of the substance to be examined in water R and dilute to 5 ml with the same solvent. Test solution (b). Dilute 1 ml of test solution (a) to 10 ml with water R. Reference ...
The most common and easy to implement method of its identification is high-performance liquid chromatography , which is described in detail in this chapter. Key words Pantothenic acid, Pantothenate , Calcium-d-pantothenate, Analysis, High-performance liquid chromatography
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This guide is engineered for senior scientists and method developers, bypassing basic overviews to directly address the physical chemistry, thermodynamic partitioning, and detection mechanics required to resolve D-calcium pantothenate co-elution.
Since pantothenic acid is quite labile to the action of high temperatures, for the enrichment of food and feed is usually used its more stable form—calcium pantothenate . The most common and easy to implement method of its identification is high-performance liquid chromatography , which is described in detail in this chapter.
PDF Calcium Pantothenate
Identification of impurities Use the chromatogram obtained with reference solution (c) to identify the peak due to impurity C; use the chromatogram supplied with pantothenate for peak identification CRS and the chromatogram obtained with reference solution (d) to identify the peaks due to impurities B, E and H.
Assay Preparation— Proceed as directed in the individual monograph for preparing a solution expected to contain approximately the equivalent of the calcium pantothenate concentration in the Standard Preparation.