This website is the central location for analyses performed by Ann Wells. This website contains the transcriptomics data analysis that accompanies the paper “Transcriptome Analysis Reveals Organ-Specific Effects of 2-Deoxyglucose Treatment in Healthy Mice”. A depiction of the experimental design is above. The experimental design contains 4 male C57BL/6J mice per treatment combination. Four mice were fed 2-deoxyglucose (2DG) and 4 others acted as a control. Nine tissues (spleen, kidney, skeletal muscle, liver, heart, small intestine (ileum), hippocampus, hypothalamus, and pre-frontal cortex) and serum were taken from each mouse. Two time points were measured (96 hours and 4 weeks) with each set of mice being euthanized after their respective time point.

For more information or to perform your own subsequent analysis please see the icons in the navigation bar to the right. There you will find the shiny app, which enables you to plot any genes of interest by tissue or treatment; the github repository, which contains the raw code; figshare, which contains the data, and a link to email Dr. Chih-Hao “Lucas” Chang and myself for any follow up questions.

Enjoy!

Analysis performed by Ann Wells

The Carter Lab The Jackson Laboratory 2023

ann.wells@jax.org